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Azco Biotech, Inc.

Solutions for Nucleic Acid Synthesis, Sequencing and Detection

Dye Colors Azco Fluorescent Detection Reagents

For many biological applications today there is a need for the ability to detect a molecule, either a protein, nucleic acid or small molecule for both in-vivo and ex-vivo applications. For this reason Azco offers a complete line of fluorescent dyes for your use in the detection of nucleic acids, proteins and other molecules of interest for a variety of biological applications.

Many biological applications today employ FRET (fluorescence resonance energy transfer) where a dye is used in conjunction with a quencher in a close proximity to one another. When the two molecules are together, fluorescence is quenched. But, when either the quencher or dye is released fluorescence is detected. This process is commonly used in many diagnostics. Azco offers a quenchers that are wavelength matched to common fluorophors to simplify FRET probe design and optimization.

Finally, Azco offers many of the dyes and quenchers we offers as phosphoramidites and CPG so that it is easy synthesize oligos that incorporate these dyes into your nucleic acid probe design.

Remember, all Azco dyes are offered for royalty free and license free use.

TF Dyes

Azco offers a series of dyes that have improved optical and chemical characteristics over many of the commonly used dyes. The name given to this family of dyes is called the TF (TideFluor) dyes. The TF family of dyes include:

TF Dye

Replaces

Ex

Em

Molar Ex Coef (cm-1 M-1)

TF1

EDANS

341

447

45,000

TF2

FAM

399

522

75,000

TF3

Cy3

554

578

135,000

TF4

ROX, Texas Red

588

610

105,000

TF5

Cy5

656

670

250,000

TF6

Cy5.5

686

702

250,000

TF7

Cy7

756

775

250,000

TF8

IRDye 800

787

808

250,000


TQ Quenchers

Since the TF dyes are developed for FRET applications, we offer a series of wavelength matched quenchers called the TQ Quenchers that quench the emission wavelengths of the complimentary dye. The dye-quencher pairs available are:

Dye

Quencher

Ex

TF1

TQ1

490

TF2

TQ2

515

TF3

TQ3

570

TF4

TQ4*

610

TF5

TQ5*

670

TF6

TQ6*

705

TF7

TQ7*

775

TF8

TQ8*

810

* denotes coming soon.

Read the TF and TQ pages for more detail about these dyes

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